NSP8_NSP7/NSP12 (SARS-CoV-2) Complex Recombinant

• Description:

  Recombinant complex of three SARS-CoV-2 proteins NSP7 (nonstructural protein 7), NSP8 (nonstructural protein 8) and NSP12 (nonstructural protein 12) . The NSP8_NSP7 construct contains an N-terminal His-tag (14xHis) followed by a TEV protease target sequence that allows cleavage of His-tag (TEV: tobacco etch virus cysteine protease BPS Bioscience #50308) . The recombinant NSP12 construct is full length encompassing amino acids 1-932 (end) . These recombinant proteins are co-expressed in an E. coli expression system and affinity purified.

• CAS Number:

  9000-83-3

• Tag:

  NSP8_NSP7: N-terminal His-tag

• Applications:

  Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

• Purity:

  ≥ 80%

• Format:

  Aqueous buffer solution

• Shipping Conditions:

  -80°C

• Storage Conditions:

  At least 6 months at -80°C.

• Calculated Molecular Weight:

  NSP12: 108 kDa; NSP8: 23 kDa; NSP7: 10 kDa

• Formulation:

  20 mM HEPES, pH 8.0,300 mM NaCl, 10% glycerol, and 3 mM DTT