NLRP3 (delPYD) / NEK7 Complex Recombinant

• Description:

  Complex of NLRP3 and NEK7 proteins. The two proteins were co-expressed and affinity purified.Recombinant human NLRP3 (NLR family pyrin domain containing 3), encompassing amino acids 134-1036 (end) . The construct does not contain the pyrin domain (also called DAPIN domain or PYD), which is involved in PYCARD binding. It contains an N-terminal FLAG-tag followed by an Avi-Tag™.Recombinant human NEK7 (NIMA related kinase 7), encompassing amino acids 2-302 (end) . This construct contains an N-terminal His-tag (7xHis) .

• UniProt:

  NLRP3: Q96P20; NEK7: Q8TDX7

• Tag:

  NLRP3: N-terminal FLAG-tag; NEK7: N-terminal His-tag

• Purity:

  ≥ 90%

• Shipping Conditions:

  -80°C

• Storage Conditions:

  At least 6 months at –80°C.

• Calculated Molecular Weight:

  NLRP3: 105 kDa; NEK7: 37 kDa

• Formulation:

  40 mM Tris-HCl pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20,100 μg/ml FLAG peptide, 3 mM DTT and 20% glycerol.