Spike S1 (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2) : ACE2 TR-FRET Assay Kit

• Background:

  The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) . As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. SARS-CoV-2 Variant B.1.1.529 BA.1, also known as Omicron variant, was originally discovered in South Africa and has recently become a global variant of concern. This variant displays increased transmissibility and infectivity compared to previous known variants.Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and human ACE2 may offer some protection against the viral infection.  The SARS-CoV-2 Spike (B.1.1.529 BA.1, Omicron Variant) protein in this kit consists of the S1 protein, which includes the ACE2 binding site. Compared to the wild-type strain, it contains mutations A67V, Δ69-70, T95I, G142D, Δ143-145, Δ211, L212I, Ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, K484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H.

• Description:

  The Spike S1 (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2) : ACE2 TR-FRET Assay is designed to measure the inhibition of the binding between SARS-CoV-2 Spike S1 (B.1.1.529 BA.1, Omicron Variant) and human ACE2 in a homogeneous 384 reaction format. This TR-FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; the test inhibitor compound is incubated with biotinylated Spike S1, Eu-labeled ACE2, and the dye-labeled acceptor for one hour. Then the TR-FRET signal is measured using a fluorescence reader capable of measuring Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET) .

• Applications:

  This kit is useful to screen for inhibitors of the interaction between SARS-CoV-2 Spike S1 (Omicron Variant) and human ACE2.

• Format:

  Catalog #NameAmountStorage100705ACE2, His-Tag, Eu-labeled*2 x 2 µg-80°C101334Spike S1 (B.1.1.529 BA.1, Omicron Variant), Avi-His-Tag, Biotin-Labeled (SARS-CoV-2) *2 x 25 µg-80°CDye-labeled acceptor3 x 10 µl-20°C799533x ACE2-Spike TR-FRET Buffer4 ml-20°C79969384-well white microplate1Room Temp*The initial concentrations of ACE2 and Spike S1 are lot-specific and will be indicated on the tubes containing the proteins.

• Shipping Conditions:

  -80°C

• Storage Conditions:

  This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

• Notes:

  Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]