Spike S1 (B.1.1.7; Alpha Variant) (SARS-CoV-2) : ACE2 TR-FRET Assay Kit

• Background:

  The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) . As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein binds to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and human ACE2 may offer some protection against the viral infection.SARS-CoV-2 (B.1.1.7), also known as Alpha variant, was first identified in the United Kingdom in the summer of 2020. This variant contains mutations N501Y, A570D, D614G, P681H, and deletions 69-70HV and 144Y in the Spike protein that may lead to higher transmissibility and infectivity.

• Description:

  The Spike S1 (B.1.1.7; Alpha Variant) (SARS-CoV-2) : ACE2 TR-FRET Assay is designed to measure the inhibition of the binding between SARS-CoV-2 Spike S1 (B.1.1.7; Alpha Variant) and human ACE2 in a homogeneous 384 reaction format. This TR-FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; the test inhibitor compound is incubated with biotinylated Spike S1, Eu-labeled ACE2, and dye-labeled acceptor for one hour. Then the TR-FRET signal is measured using a fluorescence reader capable of measuring Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET) .

• Applications:

  This kit is useful for screening for inhibitors of the interaction between SARS-CoV-2 Spike S1 (B.1.1.7; Alpha Variant) and human ACE2.

• Format:

  Catalog #NameAmountStorage100705ACE2, His-Tag, Eu-labeled*2 x 2 µg-80°C101002Spike S1* (B.1.1.7 Variant), Avi-His-tag, Biotin Labeled (16-685) (SARS-CoV-2) 25 µg-80°CDye-labeled acceptor3 x 10 µl-20°C799533x ACE2-Spike TR-FRET Buffer4 ml-20°C79969384-well white microplate1Room Temp*The initial concentration of both ACE2 and Spike S1 is lot-specific and will be indicated on the tube containing the protein.

• Shipping Conditions:

  -80°C

• Storage Conditions:

  This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

• Notes:

  Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]

• Contraindications:

  Keep final DMSO concentration at or below 1%.