XIAP, Bir2-Bir3 Domains, His-Tag Recombinant

• Background:

  The XIAP (X-linked inhibitor of apoptosis protein), also known as IAP3 (inhibitor of apoptosis protein 3) and BIRC4 (baculoviral IAP repeat-containing protein 4), is a member of the IAP family of proteins.  XIAP directly regulates caspases and suppresses apoptotic cell death pathways. XIAP has three Bir (baculovirus IAP repeat) domains, involved in binding to caspases. Bir2 inhibits caspase 3 and 7, while Bir3 is involved in caspase 9 inhibition. An increased expression level of XIAP has been observed in many cancer types, such as prostate cancer. Dysfunction of XIAP has been linked to inflammatory bowel disease, cancer, neurodegenerative and autoimmunity disorders.

• Description:

  Recombinant human XIAP (x-linked inhibitor of apoptosis protein), encompassing amino acids 120-356, corresponding to the Bir2-Bir3 (baculovirus IAP repeat) domains. This construct contains an N-terminal His-tag (6xHis) . The protein was affinity purified.

• UniProt:

  P98170

• Tag:

  N-terminal His-tag

• Applications:

  Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

• Purity:

  ≥ 80%

• Format:

  Aqueous buffer solution

• Shipping Conditions:

  -80°C

• Storage Conditions:

  At least 6 months at -80°C.

• Calculated Molecular Weight:

  28 kDa

• Formulation:

  40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, variable Imidazole, 20% glycerol, 3 mM DTT