Chicken anti Clostridium difficile Binary Toxin, Subunit A

• CAS Number:

  9007-83-4

• Host:

  Chicken

• Conjugation:

  Affinity Purified

• Type:

  Primary Antibodies

• Source:

  Chickens were immunized with highly purified Binary Toxin, A subunit, purified from Clostridium difficile. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Clostridium difficile Binary Toxin, Subunit A was immunoaffinity purified using the immunogen immobilized on a solid support.

• Applications:

  WB, ELISA

• Field of Research:

  Infectious Agents and Disease, Microbiology, Toxic Compounds

• Purification Method:

  Egg-yolk derived IgY Affinity Purified

• Assay Principle:

  This antibody has been tested in direct ELISA and Western blot. A 1:4000 dilution was an effective probe for 125 ng of C. difficile Binary Toxin A Subunit. The antibody titre, measured at the midpoint, was 1:56,000 of a 2.0 mg/mL solution (~90 ng/mL) using a 2 µg coating of C. difficile Binary Toxin, A Subunit. Optimal working dilutions should be determined for your particular assay conditions.

• Concentration:

  See vial for Concentration

• Form:

  Phosphate buffered saline‚ pH 7,2 with 0,075% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles