Anti-Phospho-CREB S133 CREB1 Antibody
CAT:
519-P00577
Size:
100 µL
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No

Anti-Phospho-CREB S133 CREB1 Antibody
- Background: The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMP-dependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
- Description: Boster Bio Anti-Phospho-CREB S133 CREB1 Antibody (Catalog # P00577). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
- Synonyms: CREB pS133, Cyclic AMP-responsive element-binding protein 1, Active transcription factor CREB, cAMP responsive element binding protein 1, CREB 1, CREB-1, CREB1, MGC9284, Transactivator protein
- CAS Number: 9007-83-4
- Gene Name: CREB1
- UniProt: P16220
- Host: Rabbit
- Reactivity: Human, Mouse, Rat
- Immunogen: CREB phospho peptide corresponding to amino acid residues 122-147 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
- Clonality: Polyclonal
- Applications: ELISA, WB
- Purification: Anti-CREB pS133 was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first sequentially pre-adsorbed against a control E.coli lysate, nickel-purified recombinant CREB and the non-phosphorylated form of the immunizing peptide. The resultant depleted antiserum was then purified against the phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS133 of human CREB. Reactivity with non-phosphorylated CREB is minimal. This antibody does show cross-reactivity with pS133 phosphorylated CREB from mouse and rat.
- Concentration: 0.064 mg/mL by UV absorbance at 280 nm
- Form: Liquid (sterile filtered)
- Molecular Weight: 36688 MW
- Components: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
- Storage Conditions: Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- Fragment: IgG
- Applications Notes: Anti-CREB pS133 antibody reacts with phosphorylated human CREB at pS133 and shows minimal reactivity by western blot with non-phosphorylated CREB and minimal reactivity (1%) by ELISA against the non-phosphorylated form of the immunizing peptide. This antibody was assayed against a variety of tissues including fibroblasts and B-cell lysates. Bands of 46 and 43 kDa corresponding to phosphorylated CREB are observed in western blots. Anti-CREB pS133 is suitable for the detection by immunoblot of phosphorylated human, mouse and rat CREB. No cross-reactivity occurs with non-phosphorylated CREB. For immunohistochemistry, formalin fixed, paraffin embedded human tissue shows moderate to strong nuclear staining in a variety of cells with minimal background staining. Although not tested this antibody is likely functional by FACS and immunoprecipitation.