Mouse SAP/PTX2/APCS PicoKine® Quick ELISA Kit

• Background:

  Amyloid P component, serum (SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.

• :

  heparin or EDTA

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	3608	4012	4067	4138	3956	205.96	5.2%
  Sample 2	12778	11342	12753	12065	12234	589.38	4.8%
  Sample 3	47613	50001	47706	49348	48667	1034.13	2.1%

  *number of samples for each test n=16.

• Description:

  Mouse SAP/PTX2 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Apcs in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Synonyms:

  Serum amyloid P-component;SAP;Apcs;Ptx2, Sap;

• CAS Number:

  7732-18-5

• Gene Name:

  Serum amyloid P-component

• Gene ID:

  20219

• UniProt:

  P12246

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: Q21-D224

• Applications:

  ELISA

• Standard:

  100 ng/tube

• Sample Type:

  cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

• Detection Range:

  1.56 ng/ml - 100 ng/ml

• Sensitivity:

  <50 pg/ml

• Components:

  Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x)|100 ul Avidin-Biotin-Peroxidase Complex (30x)|400 µl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x)|20 ml Color Developing Reagent (TMB)|10 ml Stop Solution|10 ml Plate Sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  26247 MW

• Specificity:

  Natural and recombinant mouse SAP

• Subcellular Location:

  Secreted.

• Sequence Similarities:

  Belongs to the pentaxin family.

• Protein Name:

  Serum amyloid P-component