Mouse Flt-3 Ligand ELISA Kit PicoKine®

• Background:

  Fms-related tyrosine kinase 3 ligand (FLT3LG), also known as Flt-3ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3ligand can control the development of Dendritic cells (DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	66	73	74	70	70	3.11	4.4%
  Sample 2	243	247	257	224	242	11.96	4.9%
  Sample 3	884	811	863	831	847	28.18	3.3%

  *number of samples for each test n=16.

• Description:

  Mouse Flt-3 Ligand ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Flt3lg in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Synonyms:

  Fms-related tyrosine kinase 3 ligand;Flt3 ligand;Flt3L;SL cytokine;Flt3lg;Flt3l;

• CAS Number:

  7732-18-5

• Gene Name:

  Fms-related tyrosine kinase 3 ligand

• Gene ID:

  14256

• UniProt:

  P49772

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: G27-R188

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants and serum.

• Detection Range:

  31.2 pg/ml - 2,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Function:

  Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  26141 MW

• Specificity:

  Natural and recombinant mouse Flt-3ligand

• Subcellular Location:

  Cell membrane; Single-pass type I membrane protein.

• Protein Name:

  Fms-related tyrosine kinase 3 ligand