Anti-CD49f APC

• Background:

  CD49f (alpha 6 integrin) is a type I transmembrane glycoprotein, which noncovalently associates with CD29 (beta 1 integrin) to form VLA-6, and with CD104 (beta 4 integrin) to form alpha6/beta4 integrin complex. CD49f is expressed on platelets, megakaryocytes, monocytes, T cells and thymocytes. It is widely expressed on many cultured adherent cell lines and on epithelia in non-lymphoid tissues. It is important for adhesion to laminins, invasin, and merosin, hence also for cell migration, embryogenesis, metastasis, formation of hemidesmosomes in epithelia, and other processes.

• Specifications:

  The rat monoclonal antibody GoH3 recognizes an extracellular epitope of CD49f, an alpha6 integrin subunit.

• CAS Number:

  9007-83-4

• Certification:

  RUO

• Host:

  Rat

• Species Reactivity:

  Non-human primates, Horse, Pig, Rabbit, Cow, Dog, Cat, Human, Sheep, Mouse

• Immunogen:

  mouse mammary tumor cells

• Target Antigen:

  CD49f

• Isotype:

  IgG2a

• Clone:

  GoH3

• Applications:

  Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.

• Validated Applications:

  FC

• Format:

  APC

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Martignani E, Ala U, Sheehy PA, Thomson PC, Baratta M: Whole transcriptome analysis of bovine mammary progenitor cells by P-Cadherin enrichment as a marker in the mammary cell hierarchy. Comparative Study Sci Rep. 2020 Aug 2510(1):14183., URL: https://pubmed.ncbi.nlm.nih.gov/32843665/,*Knapp DJ, Hammond CA, Aghaeepour N, Miller PH, Pellacani D, Beer PA, Sachs K, Qiao W, Wang W, Humphries RK, Sauvageau G, Zandstra PW, Bendall SC, Nolan GP, Hansen C, Eaves CJ: Distinct signaling programs control human hematopoietic stem cell survival and proliferation. Blood 2017 Jan 19129(3):307-318, URL: https://pubmed.ncbi.nlm.nih.gov/27827829/,*Karthaus WR, Iaquinta PJ, Drost J, Gracanin A, van Boxtel R, Wongvipat J, Dowling CM, Gao D, Begthel H, Sachs N, Vries RGJ, Cuppen E, Chen Y, Sawyers CL, Clevers HC: Identification of multipotent luminal progenitor cells in human prostate organoid cultures. Cell 2014 Sep 25159(1):163-175., URL: https://pubmed.ncbi.nlm.nih.gov/25201529/,*Ikeda A, Yamamoto T, Mineshiba J, Takashiba S: Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f + cells. Sci Rep. 2020 Nov 1710(1):19959., URL: https://pubmed.ncbi.nlm.nih.gov/33203957/,*Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug20(8):1174-1178. doi: 10.1038/s41592-023-01846-7., URL: https://pubmed.ncbi.nlm.nih.gov/37468619/,

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.