GABA-A Receptor Alpha 2 Antibody: FITC

GABA-A Receptor Alpha 2 Antibody: FITC

• Background: The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2).
• Description: Mouse Anti-Rat GABA-A Receptor Alpha2 Monoclonal IgG1
• Specifications: Detects ~55kDa. Does not cross-react with GABA-A Receptor Alpha1.
• Product Name Alternative: GABA A receptor subunit alpha 2 Antibody, GABRA2 Antibody, Gamma aminobutyric acid A receptor Alpha 2 Antibody, GBRA2_Human Antibody
• Synonyms: GABA-A Receptor Alpha 2 Antibody, Clone N399/19: FITC
• CAS Number: 9007-83-4
• UNSPSC: 12352203
• Gene ID: 289606
• Swiss Prot: P23576
• Accession Number: NP_001129251.1
• Cellular Locus: Cytoplasm
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Fusion protein amino acids 350-385 (Cytoplasmic C-terminus) of rat GABA-A Receptor Alpha2
• Target: GABA A Receptor
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: N399/19 (Formerly sold as S399-19)
• Conjugation: FITC
• Validated Applications: IHC, ICC/IF
• Purification: Protein G Purified
• Limit Of Detection: A 1:100 dilution of SMC-486 was sufficient for detection of GABA-A R, Alpha2 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Concentration: 1 mg/ml
• Dilution: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Weight: 0.1
• Buffer: 640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS
• Precautions: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
• References & Citations: 1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602.