FoxO4 (phospho Ser262) rabbit pAb

• Description:

  This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010],

• Synonyms:

  FOXO4; AFX; AFX1; MLLT7; Forkhead box protein O4; Fork head domain transcription factor AFX1

• Gene ID:

  4303

• UniProt:

  P98177

• Cellular Locus:

  Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human FOXO4 around the phosphorylation site of Ser262. AA range:228-277

• Clonality:

  Polyclonal

• Validated Applications:

  WB, IHC, IF, ELISA

• Stability:

  1 year

• Concentration:

  1 mg/mL

• Dilution:

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

• Molecular Weight:

  54kD

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.