Casein Kinase Iα (phospho Thr321) rabbit pAb

• Description:

  Catalytic activity: ATP + a protein = ADP + a phosphoprotein. function: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 on 'Ser-45'. similarity: Belongs to the protein kinase superfamily. similarity: Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily. similarity: Contains 1 protein kinase domain. subunit: Monomer. Interacts with the Axin complex.

• Synonyms:

  CSNK1A1; Casein kinase I isoform alpha; CKI-alpha; CK1

• Gene ID:

  1452

• UniProt:

  P48729

• Cellular Locus:

  Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Nucleus speckle. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, spindle. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis. Also recruited to the keratin cytoskeleton (PubMed:23902688)..

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human Casein Kinase I alpha around the phosphorylation site of Tyr321. AA range:287-336

• Clonality:

  Polyclonal

• Validated Applications:

  WB, IHC, IF, ELISA

• Stability:

  1 year

• Concentration:

  1 mg/mL

• Dilution:

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

• Molecular Weight:

  35kD

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.