Vimentin rabbit pAb

• Description:

  This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract. [provided by RefSeq, Jun 2009],

• Synonyms:

  VIM; Vimentin

• Gene ID:

  7431

• UniProt:

  P08670

• Cellular Locus:

  Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix. Cell membrane.

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human Vimentin. AA range:56-105

• Clonality:

  Polyclonal

• Validated Applications:

  WB, IHC, IF, ELISA

• Stability:

  1 year

• Concentration:

  1 mg/mL

• Dilution:

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

• Molecular Weight:

  50-57kD

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.