YAP (phospho Ser127) rabbit pAb

• Description:

  This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013],

• Synonyms:

  YAP1; YAP65; Yorkie homolog; 65 kDa Yes-associated protein; YAP65

• Gene ID:

  10413

• UniProt:

  P46937

• Cellular Locus:

  Cytoplasm. Nucleus. Both phosphorylation and cell density can regulate its subcellular localization (PubMed:18158288, PubMed:20048001). Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus (PubMed:18158288, PubMed:20048001). At low density, predominantly nuclear and is translocated to the cytoplasm at high density (PubMed:18158288, PubMed:20048001, PubMed:25849865). PTPN14 induces translocation from the nucleus to the cytoplasm (PubMed:22525271). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm at the blastocyst and epiblast stages (By similarity)..

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat, Monkey

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human YAP around the phosphorylation site of Ser127. AA range:93-142

• Clonality:

  Polyclonal

• Validated Applications:

  IF, WB, IHC, ELISA

• Stability:

  1 year

• Concentration:

  1 mg/mL

• Dilution:

  IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

• Molecular Weight:

  65kD

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.