Anti-FLIP (CT) Antibody

Anti-FLIP (CT) Antibody

• Description: Rabbit Polyclonal Antibody specific to FLIP (CT)
• Product Name Alternative: Caspase homolog, CASH, Caspase-eight-related protein, Casper, Caspase-like apoptosis regulatory protein, CLARP, Cellular FLICE-like inhibitory protein, c-FLIP, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, I-FLICE, MACH-related inducer of toxicity, MRIT, Usurpin [Cleaved into: CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p12]
• CAS Number: 9007-83-4
• Gene Name: CFLAR
• Gene ID: 8837
• NCBI Gene ID: CFLAR
• UniProt: O15519
• Accession Number: NP_001120655.1
• Host: Rabbit
• Reactivity: Human
• Immunogen: Peptide corresponding to aa 447- 464 at the C-terminus of human FLIPa/FLIPL (accession no. AAC51622)
• Target Antigen: CASP8 and FADD-like apoptosis regulator
• Target: FLIP (CT)
• Clonality: Polyclonal
• Isotype: IgG
• Type: Antibody
• Applications: ELISA, WB, ICC, IF
• Field of Research: Apoptosis
• Purification Method: Purified by peptide immuno-affinity chromatography
• Concentration: Lot Specific
• Dilution: Dilute in PBS or medium which is identical to that used in the assay system.
• Format: Purified
• Form: Liquid
• Buffer: Phosphate Buffered Saline
• Function: Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. {PubMed:9880531}.
• Additionnal Information: Immunoblotting: use at 1ug/mL.
  
  Immunocytochemistry: use at 10ug/mL.
  
  Positive control: Whole cell lysate from HeLa cells.
  
  These are recommended concentrations.
  
  Enduser should determine optimal concentrations for their applications.
• Storage Conditions: This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
• Specificity: This antibody recognizes full-length human, mouse, and rat FLIPalpha (55kDa).
• Formulation: PBS, pH 7.4.
• Buffer pH: pH 7.4
• Target Background: Caspase-8 (FLICE) and -10 (FLICE2) are two pivotal members of the ICE/CED-3 protease family. FLICE-inhibitory proteins have been identified in viruses and human cells and are designated v-FLIPs and FLIP, respectively. Human FLIP was cloned by several independent laboratories and designated Casper, I-FLICE, FLAME-1, CASH, and CLARP. FLIP contains two death effector domains and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and -10 and potently inhibits apoptosis induced by death receptors CD95, DR3, TRAIL-R, and TNFR1. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, g, and delta, respectively.