Anti-Glutathione-S-Transferase (GST) Antibody

Anti-Glutathione-S-Transferase (GST) Antibody

• Description: Mouse Monoclonal Antibody specific to GST
• Product Name Alternative: GST 28
• CAS Number: 9007-83-4
• UniProt: P26624
• Host: Mouse
• Reactivity: Glutathione-S-Transferase (Gst)
• Immunogen: GST protein of Schistosoma japonicum expressed in E. coli.
• Target Antigen: Glutathione S-transferase class-mu 28 kDa isozyme
• Target: Glutathione-S-Transferase (GST)
• Clonality: Monoclonal
• Isotype: IgG1
• Type: Antibody
• Applications: WB, IP
• Field of Research: Epitope Tags
• Purification Method: Purified by Protein G affinity chromatography
• Concentration: 1.0 mg/mL
• Dilution: Dilute in PBS or medium that is identical to that used in the assay system.
• Format: Purified
• Form: Liquid
• Buffer: Phosphate Buffered Saline
• Function: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.; GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
• Additionnal Information: Immunoblotting: use at 0.2-2ug/mL.
  
  These are recommended concentrations.
  
  Endusers should determine optimal concentrations for their applications.
• Storage Conditions: This antibody is stable for at least one (1) year at -20°C.
• Specificity: This antibody recognizes GST.
• Formulation: PBS, pH 7.4.
• Buffer pH: pH 7.4
• Target Background: A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids which, compared to tags such as the myc-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. Many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.