Recombinant Rat IL-28B/IFN-lambda-3 protein (His tag)

• Background:

  Interleukin-28B, also known as Cytokine Zcyto22, Interferon lambda-3, Interferon lambda-4, IFNL3, IFNL4, ZCYTO22 and IL28B, is a secreted cytokine which belongs to the IL-28/IL-29 family. IL-28 has also been shown to play a role in the adaptive immune response. IL28B has immunomodulatory activity and up-regulates MHC class I antigen expression. IL28B displays potent antiviral activity and antitumor activity. In addition, IL28B is a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway.

• Abbreviation:

  IL-28B, IFN-lambda-3

• Synonyms:

  Cytokine Zcyto; IL-12 subunit p; ZCYTO; Cytokine Zcyto22; Cytotoxic Lymphocyte Maturation Factor 35 kDa; IFNL4; IFN-Lambda-3; IFN-Lambda-4; IL-12 subunit p35; IL-12A; IL-28B; IL28C; IL-28C; Interferon Lambda-3; Interferon Lambda-4; Interleukin-12 subunit alpha; Interleukin-28B; Interleukin-28C; ZCYTO22; Il28; Il28b; Ifnl3

• UniProt:

  F1M3K4

• Accession Number:

  F1M3K4

• Expression System:

  E. coli

• Tag:

  N-His

• Sequence:

  His 19-Ala 192

• Field of Research:

  Signal Transduction; Microbiology; immunology

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  19.0 kDa

• Observed Molecular Weight:

  25 kDa

• Formulation:

  Lyophilized from sterile PBS, pH 7.4.
  Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Rat