Anti-Thymidine kinase Antibody

Anti-Thymidine kinase Antibody

• Description: Mouse Monoclonal Antibody specific to Thymidine Kinase
• Product Name Alternative: EC 2.7.1.21
• CAS Number: 9007-83-4
• Gene Name: TK1
• NCBI Gene ID: TK1
• UniProt: P04183
• Cellular Locus: Cytoplasm.
• Host: Mouse
• Reactivity: Human
• Immunogen: 5'-His-tagged recombinant human thymidine kinase.
• Target Antigen: Thymidine kinase, cytosolic
• Target: Thymidine kinase
• Clonality: Monoclonal
• Isotype: IgG1
• Type: Antibody
• Applications: WB, ELISA
• Field of Research: Cancer research
• Purification Method: Purified by Protein G affinity chromatography
• Concentration: Lot Specific
• Dilution: Dilute in PBS or medium that is identical to that used in the assay system.
• Format: Purified
• Form: Liquid
• Buffer: Phosphate Buffered Saline
• Additionnal Information: Immunoblotting: antibody at 10 ug/ml will recognize TK in 5, 10, or 20 ug lysates of cells that express TK
  
  ELISA: use antibody at 2-5 ug/ml (optimized for TK on solid phase at 10 ug/ml).
• Storage Conditions: This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
• Specificity: This antibody specifically recognizes human thymidine kinase.
• Formulation: PBS, pH 7.4.
• Buffer pH: pH 7.4
• Target Background: Thymidine kinase (TK) belongs to a group of enzymes such as dihydrofolate reductase, thymidylate synthase, and DNA polymerase that are involved in DNA synthesis and precursor production. High levels of these enzymes are present in proliferating cells, and low levels are found in resting cells, due to multiple regulatory mechanisms that ensure exclusive expression of these enzymes in replicating cells. TK is responsible for catalyzing the phosphorylation of thymidine, which functions as a part of the pyrimidine salvage pathway involved in DNA synthesis. The activities of enzymes such as TK are essential for the activation of several chemotherapeutically important nucleoside analogues that are administered as prodrugs.