Recombinant Rat RUNX2/CBFA1 protein (His tag)

• Background:

  CBFA1, also called runt-related transcription factor 2 (RUNX2), is an essential transcription factor for the regulation of osteoblast differentiation. The CBFA1 gene potentially encodes several proteins that differ in their N-terminal sequences and transactivation capacities.

• Abbreviation:

  RUNX2, CBFA1

• Synonyms:

  Acute myeloid leukemia 3 protein; CBF-alpha-1; CBFA1; CCD1; CCDAML3; CLCD; ML3; OSF-2; OSF2; PEA2-alpha A; PEA2aA; PEBP2-alpha A; PEBP2aA; RUNX2; oncogene AML-3

• UniProt:

  Q9Z2J9

• Accession Number:

  Q9Z2J9

• Expression System:

  E. coli

• Tag:

  N-His

• Sequence:

  Asp 127-Ile 218

• Field of Research:

  Epigenetics and Nuclear Signaling; Stem Cells; Developmental Biology; Neuroscience

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  10.0 kDa

• Observed Molecular Weight:

  12 kDa

• Formulation:

  Lyophilized from sterile PBS, pH 7.4.
  Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Rat