Recombinant GFP Protein

• Background:

  Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.

• Abbreviation:

  GFP

• Synonyms:

  Green fluorescent protein

• UniProt:

  P42212

• Accession Number:

  P42212

• Expression System:

  E. coli

• Tag:

  N-His

• Sequence:

  Met1-Lys238

• Field of Research:

  Tags & Cell Markers

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 90% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  26.9 kDa

• Observed Molecular Weight:

  30 kDa

• Formulation:

  Lyophilized from sterile PBS, pH 7.4,15% glycerol, 5% trehalose, 5% mannitol, 0.01% Tween 80.
  Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Aequorea victoria