Recombinant Mouse IGFBP-1 Protein (Sumo Tag)

• Background:

  IGFBP1, also known as IGFBP-1 and insulin-like growth factor-binding protein 1, is a member of the insulin-like growth factor-binding protein family. IGF binding proteins (IGFBPs) are proteins of 24 to 45 KD. All six IGFBPs share 50% homology and have binding affinities for IGF-I and IGF-II at the same order of magnitude as the ligands have for the IGF-IR. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth-promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. IGFBP1 has an IGFBP domain and a thyroglobulin type-I domain. It binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. The binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors.

• Abbreviation:

  IGFBP-1

• Synonyms:

  IBP; placental protein; IGF-BP; AFBP; IBP1; IGF-BP25; placental protein 12; PP12; Igfbp-1; Igfbp1

• UniProt:

  P47876

• Accession Number:

  P47876

• Expression System:

  E. coli

• Tag:

  N-Sumo

• Sequence:

  Ala26-Asn272

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 90% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  40.1 kDa

• Observed Molecular Weight:

  42 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

• Species:

  Mouse