Rbx1/Cul4B/Dcaf11/Ddb1 Complex Recombinant

• Background:

  Eukaryotic cells have evolved complex mechanisms to regulate cellular pathways and protein quality. Cullin proteins are a family of hybrophobic proteins that serve as scaffold and can complex with RING and adaptor proteins to form CRLs (E3 ligase-Cullin-RING ligase) complexes, which can interact with different substrates and regulate multiple cellular processes. When the CRL Rbx1-Cul4b-Ddb1 binds to Dcaf11 it can target p21 for ubiquitination and regulate cell cycle progression.

• Description:

  Complex of Rbx1 (Ring-Box 1), Cul4B (cullin 4B), Dcaf11 (Ddb1- and Cul4-associated factor 11) and Ddb1 (Damage specific DNA binding protein 1. Recombinant Rbx1, encompassing amino acids 2-108 (end), mutated at M5I, contains an N-terminal His-tag (6xHis) . Recombinant Cul4B, encompassing amino acids 3-913 (end), contains an N-terminal His-tag (6xHis) . Recombinant Dcaf11, encompassing amino acids 2-546 (end), contains an N-terminal FLAG-tag. Recombinant human Ddb1, encompassing amino acids 2-1140 (end) contains an N-terminal FLAG-tag. These recombinant proteins were co-expressed, and affinity purified.

• UniProt:

  Rbx1: P62877, Cul4B: Q13620, Dcaf11: Q8TEB1, Ddb1: Q16531

• Purity:

  ≥ 85%

• Format:

  Aqueous buffer solution.

• Shipping Conditions:

  -80°C

• Storage Conditions:

  At least 6 months at -80°C.

• Calculated Molecular Weight:

  Rbx1: 13 kDa; Cul4B: 105 kDa; Dcaf11: 63 kDa; Ddb1: 128 kDa

• Formulation:

  40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% glycerol