C-Myc: WDR5 [Biotinylated] Binding Chemiluminescent Assay Kit

• Background:

  WDR5 (WD40 repeat-containing protein 5) is a protein that is part of the histone methyltransferase complex SET1/MLL (Protein Mixed Lineage Leukemia) which catalyzes histone 3 lysine 4 methylation (H3K4me) . In addition to its direct role in gene regulation by impacting histone methylation, WDR5 can induce transcription of c-Myc induced genes, by bringing c-Myc to the appropriate regions of the chromosome. WDR5 has two protein interaction sites: the WDR5-interacting (WIN) binding site and the WDR5-binding-motif (WBM) site. MLL1 forms the complex via the WIN binding site, while RbBP5 is bound to WBM site, which is also the site for c-Myc oncoproteins interaction. WDR5 is found at high levels in certain cancers, as is c-Myc.  The addition of inhibitors that competitively bind to WIN or WBM sites has been shown to disrupt MLL activity as well as displace c-Myc from chromatin and therefore disabling its tumorigenic function.

• Description:

  The c-Myc: WDR5 [Biotinylated] Binding Chemiluminescent Assay Kit is an ELISA designed to measure the binding between c-Myc and WDR5 (WD40 repeat domain 5) protein for screening and profiling applications. This kit comes with enough purified c-Myc (amino acids 2-454 (end) ) and biotinylated WDR5 (amino acids 2-334 (end) ), MLL1 (amino acids 3745-end), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions.Figure 1. c-Myc: WDR5 [Biotinylated] Binding Chemiluminescent Assay Kitschematic.A 96-well plate is coated with c-Myc protein. After coating and blocking, MLL1 and biotinylated WDR5 are added in an optimized assay buffer. Next, unbound biotinylated WDR5-MLL complexes are washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of WDR5 binding to c-Myc.Interested in having us perform the screening and profiling of inhibitors or blocking antibodies? Visit ourMethyltransferase Screening& Profiling Services

• UniProt:

  C-Myc: P01106, WDR5: P61964

• Applications:

  Study and screen compounds that inhibit the binding of c-Myc to WDR5 for drug discovery in high throughput screening (HTS) applications.

• Format:

  Catalog #NameAmountStorage40453c-Myc, His-tag*5 µg-80°C80016WDR5, His-Tag, Biotin-Labeled *5 µg-80°C79186MLL1, His-Tag*5 µg-80°C82952PP-03 Buffer10 ml-20°C79728Blocking Buffer 225 ml+4°C79742Streptavidin HRP10 µl+4°C79670ELISA ECL Substrate A (translucent bottle) 6 mlRoom TempELISA ECL Substrate B (brown bottle) 6 mlRoom Temp79699White 96-well microplate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

• Shipping Conditions:

  -80°C

• Storage Conditions:

  This assay kit will perform optimally for up to6 months from date of receipt when the materials are stored as directed.

• Notes:

  Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected].

• Contraindications:

  This assay kit is compatible with up to 1% final DMSO concentration.