Rat Anti-Mouse CD49e-FITC

• CAS Number:

  9007-83-4

• Reactivity:

  Mouse

• Storage Conditions:

  The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 °C.
  The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 °C.
  The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 °C.
  The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 °C. Do not freeze!
  The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 °C.
  Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

• Specificity:

  alpha 5 subunit of the integrin alpha 5 beta 1 fibronectin receptor
  The monoclonal antibody 5H10-27 reacts with the alpha 5 chain (VLA-5) of the CD49e/CD29 heterodimeric fibronectin receptor. It is expressed on thymocytes, activated T cells, mast cells, and a variety of mouse cell lines.^1-5 Soluble 5H10-27 antibody inhibits VLA-5-mediated functions in vitro.^1, 2-4, 6 Immobilized 5H10-27 has also been demonstrated to co-stimulate the proliferative response of CD8^+ T cells to plate-bound anti- CD3 monoclonal antibody.^3

• Short Name:

  [CD49e/VLA-5]