(KO Validated) HIF1AN Polyclonal Antibody

• Background:

  Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD. Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.

• Synonyms:

  HIF1AN,FIH1

• Gene ID:

  55662

• Swiss Prot:

  Q9NWT6

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Immunogen:

  Recombinant fusion protein of human HIF1AN

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Applications:

  WB,IF

• Purification:

  Affinity purification

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:500-1:2000,IF 1:50-1:200

• Buffer:

  PBS with 0.05% proclin300,50% glycerol,pH7.3.

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  14kDa

• Observed Molecular Weight:

  14kDa/16kDa