Anti-FDX1 | Ferredoxin

Anti-FDX1 | Ferredoxin

• Background: Ferredoxinsare acidic, low molecular weight, soluble iron-sulfur proteins found in various organisms. Iron-sulfur proteins are defined as proteins carrying iron-sulfur cluster(s) in which the iron is at least partially coordinated by sulfur. The protein acts as multifunctional electron carriers in diverse redox systems. The chloroplast ferredoxin is involved in both cyclic and non-cyclic photophosphorylation reactions of photosynthesis and other reductive reactions in the chloroplast.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana,Chlamydomonas reinhardtii,Hordeum vulgare,Pinus strobus,Spinacia oleracea, Synechocystis 6803substrain PCC-M
• Not reactive in: Phaeodactylum tricornutum
• Immunogen: Native ferredoxin purified fromSpinacia oleracea.
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 1000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 10 (Spinacia oleracea); 16.7 (Arabidopsis thaliana), 13.7 (Chlamydomonas reinhardtii)
• Precautions: Load per well: 20-40  µg/lane required forArabidopsis thaliana, 2-10 µg for other species.This product can be sold containing proclin if requested.
• References & Citations: Tiwariet al. (2024).Differential FeS cluster photodamage plays a critical role in regulating excess electron flow through photosystem I. Nat Plants. 2024 Oct;10(10):1592-1603. doi: 10.1038/s41477-024-01780-2.Linet al. (2024). Hydrogen production in the Chlorella sp. DT mutants carrying heterologous electron donor ferredoxin 1 of Chlamydomonas reinhardtii. Electronic Journal of Biotechnology Volume 69, May 2024, Pages 11-20.Cvetkovskaet al. (2018). Characterization of photosynthetic ferredoxin from the Antarctic alga Chlamydomonas sp. UWO241 reveals novel features of cold adaptation. New Phytol. 2018 Jul;219(2):588-604. doi: 10.1111/nph.15194.Jokelet al. (2018). Hunting the main player enabling Chlamydomonas reinhardtii growth under fluctuating light. Plant J. 2018 Mar 25. doi: 10.1111/tpj.13897.Georget al. (2017). Acclimation of Oxygenic Photosynthesis to Iron Starvation Is Controlled by the sRNA IsaR1. Curr Biol. 2017 May 22;27(10):1425-1436.e7. doi: 10.1016/j.cub.2017.04.010.Huet al. (2017). The SUFBC2 D Complex is Required for the Biogenesis of All Major Classes of Plastid Fe-S Proteins. Plant J. 2017 Jan 19. doi: 10.1111/tpj.13483.Higuchiet al. (2011). Modulation of macronutrient metabolism in barley leaves under iron-deficient condition. Soil Sc and Plant Nutr. 57 (2): 233-247.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.