PCNA Polyclonal Antibody

PCNA Polyclonal Antibody

• Background: Proliferating Cell Nuclear Antigen,commonly known as PCNA,is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase,but not apurinic-apyrimidinic (AP) endonuclease,APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells.
• Synonyms: ATLD2,cb16,Cyclin,DNA polymerase delta auxiliary protein,etID36690.10,fa28e03,fb36g03,HGCN8729,MGC8367,Mutagen-sensitive 209 protein,OTTHUMP00000030189,OTTHUMP00000030190,PCNA,Pcna/cyclin,PCNA,PCNAR,Polymerase delta accessory protein,Proliferating cell nuclear antigen,wu:fa28e03,wu:fb36g03
• CAS Number: 9007-83-4
• Swiss Prot: Q12004,P17918,P04961
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Immunogen: Recombinant protein corresponding to Mouse PCNA
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Applications: WB,IHC
• Purification: Affinity purification
• Concentration: 470 μg/mL
• Dilution: WB 1:500-1:2000, IHC 1:500-1:1000
• Buffer: PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4
• Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa