Histone H3 Polyclonal Antibody

Histone H3 Polyclonal Antibody

• Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
• Synonyms: H3.4,H3/g,H3FT,H3t,HIST3H3,Histone H3,HIST1H3A
• CAS Number: 9007-83-4
• Gene ID: 8290
• Swiss Prot: Q16695
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Immunogen: A synthetic peptide of human HIST3H3 (NP_003484.1).
• Isotype: IgG
• Conjugation: Unconjugated
• Applications: WB,IHC
• Purification: Affinity purification
• Concentration: 1 mg/mL
• Dilution: WB 1:500-1:2000 IHC 1:50-1:200
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 17 kDa