Anti-PCNA Antibody

Anti-PCNA Antibody

• Background: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
• Description: Boster Bio Anti-PCNA Antibody (Catalog # M00125-2). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
• Synonyms: Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
• Gene Name: PCNA
• UniProt: P12004
• Host: Mouse
• Reactivity: Human
• Immunogen: This PCNA antibody is generated from a mouse immunized with a recombinant protein of human PCNA.
• Clonality: Monoclonal
• Applications: WB, Flow Cytometry, IHC-P
• Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Molecular Weight: 28769 Da
• Components: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Storage Conditions: Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Fragment: IgG1, k
• Clone ID: Clone: 1655CT506.10.26