Mouse CCL8/MCP-2 ELISA Kit PicoKine®, Carrier-free

• Background:

  Chemokine (C-C motif) ligand 8, also known as monocyte chemoattractant protein 2 (MCP-2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. Monocyte chemotactic protein 2 (MCP-2) is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.

• :

  heparin or EDTA

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	37	36	34	32	34	1.92	5.6%
  Sample 2	190	188	192	174	186	7.07	3.8%
  Sample 3	475	541	471	504	497	28.03	5.6%

  *number of samples for each test n=16.

• Description:

  Mouse CCL8/MCP-2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Synonyms:

  C-C motif chemokine 8;Monocyte chemoattractant protein 2;Monocyte chemotactic protein 2;MCP-2;Small-inducible cytokine A8;Ccl8;Mcp2, Scya8;

• Gene Name:

  C-C motif chemokine 8

• Gene ID:

  20307

• UniProt:

  Q9Z121

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: E20-P97

• Applications:

  ELISA

• Standard:

  1 ng/tube

• Sample Type:

  cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

• Detection Range:

  15.6 pg/ml - 1,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Function:

  Chemotactic factor that attracts monocytes. This protein can bind heparin (By similarity). .

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  11017 MW

• Specificity:

  Natural and recombinant mouse CCL8

• Subcellular Location:

  Secreted .

• Protein Name:

  C-C motif chemokine 8