Anti-Laminin 2 alpha/LAMA2 Antibody Picoband®, APC Conjugate

Anti-Laminin 2 alpha/LAMA2 Antibody Picoband®, APC Conjugate

• Background: Laminin, alpha-2, also known as LAMA2, is a protein that in humans is encoded by the LAMA2 gene. This gene is mapped to 6q22.33. Laminin, an extracellular protein, is a major component of the basement membrane. It is though to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene.
• Description: Boster Bio Anti-Laminin 2 alpha/LAMA2 Antibody catalog # PA2160. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Laminin subunit alpha-2;Laminin M chain;Laminin-12 subunit alpha;Laminin-2 subunit alpha;Laminin-4 subunit alpha;Merosin heavy chain;LAMA2;LAMM;
• Gene Name: Laminin subunit alpha-2
• UniProt: P24043
• Host: Rabbit
• Reactivity: Rat,Mouse,Human
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Laminin 2 alpha, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
• Clonality: Polyclonal
• Tissue Specificity: Placenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain; not in liver, thymus and bone.
• Applications: WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
• Components: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• References & Citations: 1. Coral-Vazquez, R. M., Rosas-Vargas, H., Meza-Espinosa, P., Mendoza, I., Huicochea, J. C., Ramon, G., Salamanca, F. Severe congenital muscular dystrophy in a Mexican family with a new nonsense mutation (R2578X) in the laminin alpha-2 gene. J. Hum. Genet. 48: 91-95, 2003. 2. Millay, D. P., Sargent, M. A., Osinska, H., Baines, C. P., Barton, E. R., Vuagniaux, G., Sweeney, H. L., Robbins, J., Molkentin, J. D. Genetic and pharmacologic inhibition of mitochondrial-dependent necrosis attenuates muscular dystrophy. Nature Med. 14: 442-447, 2008.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 343905 MW
• Observed Molecular Weight: 14 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
  
• Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane. Major component.
• Sequence Similarities: Contains 17 laminin EGF-like domains.
• Protein Name: Laminin subunit alpha-2