Anti-STK3/MST-2 Antibody Picoband®, FITC Conjugate

• Background:

  Serine/threonine-protein kinase 3 is an enzyme that in humans is encoded by the STK3 gene. This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.

• Description:

  Boster Bio Anti-STK3/MST-2 Antibody Picoband® catalog # A02224-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Cytokine receptor common subunit beta; GM-CSF/IL-3/IL-5 receptor common beta subunit; CD131; Csf2rb; Aic2b; Csf2rb1; Il3rb1

• Gene Name:

  colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)

• Gene ID:

  6788

• UniProt:

  Q13188

• Host:

  Rabbit

• Reactivity:

  Human,Monkey

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human STK3/MST-2 recombinant protein (Position: E314-D456).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Broadly expressed with higher expression in heart.

• Applications:

  ELISA,Flow Cytometry,IF,IHC,ICC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  High affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.

• Components:

  Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

• References & Citations:

  1. Creasy, C. L., Chernoff, J. Cloning and characterization of a member of the MST subfamily of Ste20-like kinases. Gene 167: 303-306, 1995. 2. Du, X., Wen, J., Wang, Y., Karmaus, P. W. F., Khatamian, A., Tan, H., Li, Y., Guy, C., Nguyen, T.-L. M., Dhungana, Y., Neale, G., Peng, J., Yu, J., Chi, H. Hippo/Mst signalling couples metabolic state and immune function of CD8-alpha+ dendritic cells. Nature 558: 141-145, 2018. 3. Harvey, K. F., Pfleger, C. M., Hariharan, I. K. The Drosophila Mst ortholog, hippo, restricts growth and cell proliferation and promotes apoptosis. Cell 114: 457-467, 2003.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  38160 MW

• Observed Molecular Weight:

  56 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.1-0.25μg/ml, Human, Monkey
  Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
  Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5μg/ml, -
  

• Subcellular Location:

  Membrane. Single-pass type I membrane protein.

• Protein Name:

  Cone-rod homeobox protein