Anti-Mesothelin/MSLN Antibody Picoband®, HRP Conjugate

Anti-Mesothelin/MSLN Antibody Picoband®, HRP Conjugate

• Background: Mesothelin, also known as MSLN or MPF is a protein that in humans is encoded by the MSLN gene. This gene is mapped to 16p13.3. This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas.
• Description: Boster Bio Anti-Mesothelin/MSLN Antibody Picoband® catalog # PB9287. Tested in ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Mesothelin;CAK1 antigen;Pre-pro-megakaryocyte-potentiating factor;Megakaryocyte-potentiating factor;MPF;Mesothelin, cleaved form;MSLN;MPF;
• Gene Name: Mesothelin
• Gene ID: 10232
• UniProt: Q13421
• Host: Rabbit
• Reactivity: Human
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: E.coli-derived human Mesothelin recombinant protein (Position: K306-L576). Human Mesothelin shares 57% and 56% amino acid (aa) sequence identity with mouse and rat Mesothelin, respectively.
• Clonality: Polyclonal
• Tissue Specificity: Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). .
• Applications: ELISA,Flow Cytometry,IHC,IHC-F,ICC,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Membrane-anchored forms may play a role in cellular adhesion.
• Components: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• References & Citations: 1. Bera, T. K., Pastan, I. Mesothelin is not required for normal mouse development or reproduction. Molec. Cell. Biol. 20: 2902-2906, 2000. 2. Scholler, N., Fu, N., Yang, Y., Ye, Z., Goodman, G. E., Hellstrom, K. E., Hellstrom, I. Soluble member(s) of the mesothelin/megakaryocyte potentiating factor family are detectable in sera from patients with ovarian carcinoma. Proc. Nat. Acad. Sci. 96: 11531-11536, 1999.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 68986 MW
• Observed Molecular Weight: 69 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: ELISA, 0.1-0.5μg/ml, -
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
  Western blot, 0.1-0.5μg/ml, Human
  Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
  Immunocytochemistry, 0.5-1μg/ml, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
• Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus.
• Protein Name: Mesothelin