Anti-GLB1/Beta-galactosidase Antibody Picoband®, Dylight550 Conjugate

Anti-GLB1/Beta-galactosidase Antibody Picoband®, Dylight550 Conjugate

• Background: Galactosidase, beta 1, also known as GLB1, is a protein which in humans is encoded by the GLB1 gene. I t is mapped to 3p22.3. This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene may result in GM1-gangliosidosis and Morquio B syndrome.
• Description: Boster Bio Anti-GLB1/Beta-galactosidase Antibody Picoband® catalog # A01829-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Beta-galactosidase; Glb1; Glb1_mapped; rCG_25043
• Gene Name: galactosidase, beta 1
• Gene ID: 12091
• UniProt: D3ZUM4
• Host: Rabbit
• Reactivity: Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E.coli-derived rat GLB1/Beta-galactosidase recombinant protein (Position: R103-I646).
• Clonality: Polyclonal
• Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.EC:3.2.1.23
• Components: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• References & Citations: 1. Shows TB, Scrafford-Wolff L, Brown JA, Meisler M (1978). "Assignment of a beta-galactosidase gene (beta GALA) to chromosome 3 in man". Cytogenetics and Cell Genetics. 22 (1–6): 219–22. 2. Oshima A, Tsuji A, Nagao Y, Sakuraba H, Suzuki Y (Nov 1988). "Cloning, sequencing, and expression of cDNA for human beta-galactosidase". Biochemical and Biophysical Research Communications. 157 (1): 238–44. 3. Caciotti A, Donati MA, Boneh A, d'Azzo A, Federico A, Parini R, Antuzzi D, Bardelli T, Nosi D, Kimonis V, Zammarchi E, Morrone A (Mar 2005). "Role of beta-galactosidase and elastin binding protein in lysosomal and nonlysosomal complexes of patients with GM1-gangliosidosis". Human Mutation. 25 (3): 285–92.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 18080 MW
• Observed Molecular Weight: 65 kDa, 76 kDa, 85 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot, 0.25-0.5μg/ml, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat
  Immunocytochemistry/Immunofluorescence, 2μg/ml, Rat
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Mouse, Rat
  ELISA, 0.1-0.5μg/ml,
  
• Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor.
• Protein Name: Thy-1 membrane glycoprotein