Anti-AP2 gamma/TFAP2C Antibody Picoband®, Cy3 Conjugate

• Background:

  Transcription factor AP-2 gamma also known as AP2-gamma is a protein that in humans is encoded by the TFAP2C gene. The protein encoded by this gene is a sequence-specific DNA-binding transcription factor involved in the activation of several developmental genes. The encoded protein can act as either a homodimer or heterodimer with other family members and is induced during retinoic acid-mediated differentiation. It plays a role in the development of the eyes, face, body wall, limbs, and neural tube.

• Description:

  Boster Bio Anti-AP2 gamma/TFAP2C Antibody Picoband® catalog # A01558-2. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Structural maintenance of chromosomes protein 6; SMC protein 6; SMC-6; hSMC6; SMC6; SMC6L1

• Gene Name:

  structural maintenance of chromosomes 6

• Gene ID:

  7022

• UniProt:

  Q92754

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human AP2 gamma/TFAP2C recombinant protein (Position: M1-N219).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Widely expressed (PubMed:11408570). Strongly expressed in testis (PubMed:11408570).

• Applications:

  WB,IHC,ICC,IF,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. SMC5-SMC6 complex may prevent transcription of episomal DNA, such as circular viral DNA genome (PubMed:26983541).

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Aldred, M. A., Aftimos, S., Hall, C., Waters, K. S., Thakker, R. V., Trembath, R. C., Brueton, L. Constitutional deletion of chromosome 20q in two patients affected with Albright hereditary osteodystrophy. Am. J. Med. Genet. 113: 167-172, 2002. 2. Bosher, J. M., Williams, T., Hurst, H. C. The developmentally regulated transcription factor AP-2 is involved in c-erbB-2 overexpression in human mammary carcinoma. Proc. Nat. Acad. Sci. 92: 744-747, 1995. 3. Genevieve, D., Sanlaville, D., Faivre, L., Kottler, M.-L., Jambou, M., Gosset, P., Boustani-Samara, D., Pinto, G., Ozilou, C., Abeguile, G., Munnich, A., Romana, S., Raoul, O., Cormier-Daire, V., Vekemans, M. Paternal deletion of the GNAS imprinted locus (including Gnasxl) in two girls presenting with severe pre- and post-natal growth retardation and intractable feeding difficulties. Europ. J. Hum. Genet. 13: 1033-1039, 2005.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Observed Molecular Weight:

  49 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Human
  Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
  Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
  ELISA, 0.1-0.5 μg/ml, -
  

• Subcellular Location:

  Nucleus.