Anti-GABA Transporter 1/GAT 1/SLC6A1 Antibody Picoband®, Biotin Conjugate

Anti-GABA Transporter 1/GAT 1/SLC6A1 Antibody Picoband®, Biotin Conjugate

• Background: GABA transporter 1 (GAT1), also known as sodium- and chloride-dependent GABA transporter 1, is a protein that in humans is encoded by the SLC6A1 gene. GABA Transporter 1 uses Na+ and Cl- to create a gradient, which removes or adds GABA to extracellular spaces in the cerebrum and cerebellum. The stoichiometry for GABA Transporter 1 is 2 Na+: 1 Cl-: 1 GABA. The activity of GAT1 is largely dependent on the presence of Na+, while Cl- assists by increasing the ability for GAT-1 to uptake GABA.
• Description: Boster Bio Anti-GABA Transporter 1/GAT 1/SLC6A1 Antibody catalog # PA1721. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Sodium- and chloride-dependent GABA transporter 1;GAT-1;Solute carrier family 6 member 1;SLC6A1;GABATR, GABT1, GAT1;
• Gene Name: Sodium- and chloride-dependent GABA transporter 1
• UniProt: P30531
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GABA Transporter 1, identical to the related rat and mouse sequences.
• Clonality: Polyclonal
• Tissue Specificity: Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart. .
• Applications: IHC,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
• Components: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• References & Citations: 1. Carvill, G. L., McMahon, J. M., Schneider, A., Zemel, M., Myers, C. T., Saykally, J., Nguyen, J., Robbiano, A., Zara, F., Specchio, N., Mecarelli, O., Smith, R. L., and 13 others. Mutations in the GABA transporter SLC6A1 cause epilepsy with myoclonic-atonic seizures. Am. J. Hum. Genet. 96: 808-815, 2015. Huang, F., Shi, L. J., 2. Cope, D. W., Di Giovanni, G., Fyson, S. J., Orban, G., Errington, A. C., Lorincz, M. L., Gould, T. M., Carter, D. A., Crunelli, V. Enhanced tonic GABA-A inhibition in typical absence epilepsy. Nature Med. 15: 1392-1398, 2009. 3. Heng, H. H. Q., Fei, J., Guo, L.-H. Assignment of the human GABA transporter gene (GABATHG) locus to chromosome 3p24-p25. Genomics 29: 302-304, 1995.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 67074 MW
• Observed Molecular Weight: 29 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse , By Heat
  Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
  
• Subcellular Location: Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons (By similarity). .
• Sequence Similarities: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
• Protein Name: Sodium- and chloride-dependent GABA transporter 1(GAT-1)