Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMPPRTVIL) Complex Protein (Monomer, MALS verified)

• Background:

  HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin) . The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.

• Description:

  Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMPPRTVIL) Complex Protein (HL0-H82E8) is expressed from human 293 cells (HEK293) . It contains AA Gly 22 - Ile 305 (HLA-E*01:03) & Ile 21 - Met 119 (B2M) & VMPPRTVIL peptide (Accession # P13747 (HLA-E*01:03) & P61769-1 (B2M) & VMPPRTVIL) .

• Specifications:

  Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMPPRTVIL) Complex Protein is produced by co-expression of HLA and B2M loaded with CMV UL40 peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 36.2 kDa and 13.8 kDa. The protein migrates as 40-43 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  P13747 (HLA-E*01:03) & P61769-1 (B2M) & VMPPRTVIL

• Expression Region:

  Gly 22 - Ile 305 (HLA-E*01:03) & Ile 21 - Met 119 (B2M) & VMPPRTVIL peptide

• Host:

  HEK293

• Target:

  HLA-E*0103 & B2M & CMV UL40 (VMPPRTVIL)

• Conjugation:

  Biotin-labeled

• Tag:

  C-10xHis & C-Avi

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  36.2 kDa and 13.8 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  25ug*1

• Species:

  Human