Recombinant SARS-CoV-2 NSP12 protein

• Background:

  The RdRp of SARS-CoV-2 is composed of a catalytic subunit known as nsp12 as well as two accessory subunits, nsp8 and nsp7. The structure of this RdRp has recently been reported; it is highly similar to the RdRp of SARS-CoV, a zoonotic coronavirus that spread into the human population in 2002. The nsp12 subunit contains an N-terminal nidovirus RdRp-associated nucleotidyltransferase (NiRAN) domain, an interface domain and a C-terminal RdRp domain. The RdRp domain resembles a right hand, comprising the fingers, palm and thumb subdomains that are found in all single-subunit polymerases. Subunits nsp7 and nsp8 bind to the thumb, and an additional copy of nsp8 binds to the fingers domain. Structural information is also available for nsp8–nsp7 complexes.

• Synonyms:

  RNA-directed RNA polymerase; RdRp; SARS-CoV 2 nsp12

• UniProt:

  P0DTC2

• Accession Number:

  QHD43415.1

• Expression System:

  E. coli

• Tag:

  N-Gst

• Sequence:

  Asp4891-Val5212

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 90 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  64.0 kDa

• Observed Molecular Weight:

  60 kDa

• Formulation:

  Supplied as solution form in PBS, pH7.5 or lyophilized from PBS, pH7.5
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  SARS-CoV-2