Recombinant Mouse Neurotrophic Tyrosine Kinase Receptor Type 2/TrkB/NTRK2 (C-6His)

• Background:

  The TRK Family of Tyrosine Kinase Receptor consists of 3 members: TrkA, TrkB and TrkC. The three TRK family proteins have different ligand specificities. They connect to different neurotrophins, including NGF, BDNF, NT-3NT-4/5. TRKA binds NGF, TRKB binds BDNF and NT-3, TRKC binds NT-4/5. At the protein sequence level, human and rat TRKB have greater than 90% sequence identity and the proteins exbihit cross-species activity. TRKB is primarily expressed in the nervous system and it also expression in a wide variety of tissues with low levels.

• Abbreviation:

  TrkB, NTRK2

• Synonyms:

  BDNF/NT-3 Growth Factors Receptor; GP145-TrkB; NTRK2; Neurotrophic Tyrosine Kinase Receptor Type 2; TRKB; Trk-B; TrkB Tyrosine Kinase; Tropomyosin-Related Kinase B

• UniProt:

  P15209

• Accession Number:

  P15209

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Cys32-His429

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  45.3 kDa

• Observed Molecular Weight:

  60 - 90 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse