Recombinant Mouse Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-6His)

• Background: Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO--terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts
• Abbreviation: Siglec-15, CD33L3
• Synonyms: 425O18-1; Angiopoietin-like protein 4; Angiopoietin-related protein 4; Angptl4; Farp; Fasting-induced adipose factor; Fiaf; HFARP; Hepatic fibrinogen/angiopoietin-related protein; Ng27; Secreted protein Bk89
• UniProt: A7E1W8
• Accession Number: A7E1W8
• Expression System: HEK293 Cells
• Tag: C-His
• Sequence: Arg24-Thr262
• Applications: BLI
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Bioactivity: Loaded Anti-Human Siglec15 mAb-mFc on AMQ Biosensor, can bind Mouse Siglec-15-His with an affinity constant of 0.33 nM as determined in BLI assay.
• Reconstitution: Please refer to the printed manual for detailed information.
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
• Calculated Molecular Weight: 26.5 kDa
• Observed Molecular Weight: 30 - 40 kDa
• Formulation: Lyophilized from a 0.2 µm filtered solution of PBS, 150mM NaCl, 5% Thehalose, 0.3% Chaps, pH 7.4.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.
• Species: Mouse