Recombinant Mouse Interleukin-2/IL-2 Protein

• Background:

  Interleukin 2 (IL 2), also termed T-cell growth factor, is a member of the cytokine family which includes IL-4, IL-7, IL-9, IL-15 and IL-21. Each member of this family has a four alpha helix bundle. IL-2 signals through the IL-2 receptor, a complex consisting of tree subunits, termed alpha, beta and gamma. The IL-2 R gamma is shared by cytokine receptors of all members of cytokine family. Mature mouse IL-2 shares 56% and 73% aa sequence identity with human and rat IL-2, respectively. IL-2 is produced by CD4+ T cell, CD8+ T cells, gamma δ T cells, B cells, dendritic cells and eosinophils, and plays a vital role in key function of the immune system, tolerance and immunity, primarily via its potent stimulatory activity for T cells.Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity.

• Abbreviation:

  Interleukin-2, IL-2

• Synonyms:

  POIL; OTTHUMP; Aldesleukin; IL 2; Interleukin 2; Involved in regulation of T cell clonal expansion; Lymphokine; OTTHUMP00000164090; POIL2; T Cell Growth Factor; T-cell growth facter; Interleukin-2; IL2; TCGF; T-cell growth factor; Il-2

• UniProt:

  P04351

• Accession Number:

  P04351

• Expression System:

  E. coli

• Tag:

  None

• Sequence:

  Ala21-Gln169

• Applications:

  Cell culture

• Endotoxin:

  < 0.01 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells.The specific activity of Recombinant Mouse IL-2 is ≥1x10⁷ IU/mg.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  17.4 kDa

• Observed Molecular Weight:

  17 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 20mM Sodium Citrate, 0.2% Tween 80, pH 3.0.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse