Recombinant Mouse MAG/Siglec-4a Protein (His Tag)

• Background:

  Myelin-Associated Glycoprotein (MAG, Siglec-4a), is a type I transmembrane glycoprotein belonging to the Siglec family. It is composed of an extracellular segment containing five Ig-like domains, a single transmembrane segment, and a cytoplasmic domain. Mouse MAG shares 95% and 99% aa sequence identity with human and rat MAG, respectively. MAG functions as an adhesion molecule during neural development. It preferentially binds to alpha -2,3-linked sialic acid terminal structures found on cell surface molecules. MAG is selectively expressed by myelinating oligodendrocytes and Schwann cells and plays an important role in axon-myelin stability. MAG is also reported to regulate the axon cytoskeleton and support the distribution of axon molecules at the nodes of Ranvier. In addition, it has been identified as a major inhibitor of neurite outgrowth.

• Abbreviation:

  MAG, Siglec-4a

• Synonyms:

  MAG; Myelin-Associated Glycoprotein; Siglec-4a

• UniProt:

  P20917

• Accession Number:

  P20917

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Gly20-Pro516

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  55.7 kDa

• Observed Molecular Weight:

  70 - 120 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 20mM HEPES, 150mM NaCl, 1mM EDTA, pH7.0.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse