Recombinant Mouse TGFβ1/TGFB1 Protein

• Background:

  Transforming growth factor beta 1 (TGFβ1) is the prototype of a growing superfamily of peptide growth factors and plays a prominent role in a variety of cellular processes, including cell-cycle progression, cell differentiation, reproductive function, development, motility, adhesion, neuronal growth, bone morphogenesis, wound healing, and immune surveillance. TGF-β1, TGF-β2 and TGF-β3 signal via the same heteromeric receptor complex, consisting of a ligand binding TGF-β receptor type II (TβR-II), and a TGF-β receptor type I (TβR-I) . Signal transduction from the receptor to the nucleus is mediated via SMADs. TGF-β expression is found in cartilage, bone, teeth, muscle, heart, blood vessels, haematopoitic cells, lung, kidney, gut, liver, eye, ear, skin, and the nervous system.

• Abbreviation:

  TGFβ1, TGFB1

• Synonyms:

  CED; CEDLAP; DPD1; TGF-b1; TGF-beta 1 protein; TGF-beta-1; TGFB; TGFB1; TGFbeta; latency-associated peptide; transforming growth factor beta-1

• UniProt:

  P04202

• Accession Number:

  P04202

• Expression System:

  HEK293 Cells

• Tag:

  None

• Sequence:

  Ala279-Ser390

• Applications:

  Cell culture

• Endotoxin:

  < 0.01 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured by its ability to inhibit IL-4-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 5-25 pg/ml.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  12.8 kDa

• Observed Molecular Weight:

  13 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 4mM HCl.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse