Recombinant Mouse Semaphorin-4C/SEMA4C Protein (Fc Tag)

• Background:

  Semaphorin-4C is a protein which belongs to the semaphorin family, contains 1 Ig-like C2-type domain, 1 PSI domain, 1 Sema domain. As cell surface receptor for PLXNB2, it plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. It required for normal brain development, axon guidance and cell migration, Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.

• Abbreviation:

  Semaphorin-4C, SEMA4C

• Synonyms:

  M-Sema F; S4c; Sema I; Sema4c; Semacl1; Semai; Semaphorin I; Semaphorin-4C; Semaphorin-C-like 1

• UniProt:

  Q64151

• Accession Number:

  Q64151

• Expression System:

  HEK293 Cells

• Tag:

  C-Fc

• Sequence:

  Ala21-Gly664

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  99.3 kDa

• Observed Molecular Weight:

  115 - 125 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse