Recombinant Mouse Interleukin-13/IL-13 Protein

• Background:

  Mouse interleukin 13 (mIL-13) is a pleiotropic cytokine produced by activated Th2 cells. IL-13 induces B cell proliferation and immunoglobin production. It contains a four helical bundle with two internal disulfide bonds. Mouse IL13 shares 58% sequence identity with human protein and exhibits cross-species activity. IL13 signals via receptor IL13R (type2, IL4R) and activates STAT-6. IL13 initially binds IL-13Rα1 with low affinity and triggers association of IL4Rα, generating a high affinity heterodimeric receptor IL13R and eliciting downstream signals. IL13 also binds IL-13Rα2 with high affinity, which plays a role in a negative feedback system of IL13 signaling. IL13 is an important mediator of allergic inflammation and disease.

• Abbreviation:

  Interleukin-13, IL-13

• Synonyms:

  BHR; Interleukin; T-Cell Activation Protein P; ALRH; BHR1; T-Cell Activation Protein P600; Interleukin-13; IL13; NC30; P600; Il-13

• UniProt:

  P20109

• Accession Number:

  P20109

• Expression System:

  E. coli

• Tag:

  None

• Sequence:

  Ser26-Phe131

• Applications:

  Cell culture

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 1. 93 ng/ml.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  11.7 kDa

• Observed Molecular Weight:

  9 - 14 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 20mM Histidine-HCl, 8% Trehalose, 4% Mannitol, 50mM NaCl, 0.05% Tween 80, pH 6.0.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse