Recombinant Mouse Interleukin-10/IL-10 Protein

• Background: Mouse Il10 is the prototypic member of the IL-10 cytokine family, including IL-10, IL-19, IL-20, IL-22 (IL-TIF), IL-24 and IL-26. Many viruses encode viral members of the IL-10 family, such as Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) .Its main function is inhibiting the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Although human and mouse IL-10 are 81% identical at the nucleotide and amino acid level, mouse IL-10 is species-specific and does not act on human cells. Interestingly, Human IL-10 is active on mouse cells.
• Abbreviation: Interleukin-10, IL-10
• Synonyms: Interleukin; IL-; CSIF; If2a; Il-10; IL10A; TGIF; Interleukin-10; IL10; Cytokine synthesis inhibitory factor; N/A; CSIF; If2a; Il-10
• UniProt: P18893
• Accession Number: P18893
• Expression System: E. coli
• Tag: C-His
• Sequence: Ser19-Ser178
• Applications: Cell culture
• Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by reducing SDS-PAGE.
• Bioactivity: Measure by its ability to induce MC/9‑2 cells proliferation.The ED₅₀ for this effect is <1 ng/mL.The specific activity of recombinant mouse IL-10 is > 1 x 10⁶ IU/mg.
• Reconstitution: Please refer to the printed manual for detailed information.
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
• Calculated Molecular Weight: 19.7 kDa
• Observed Molecular Weight: 19 kDa
• Formulation: Lyophilized from sterile PBS, pH 7.4.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.
• Species: Mouse