Recombinant Mouse Interferon γ/IFNG Protein (E.coli)

• Background:

  Mouse Ifng is a secreted protein which belongs to the type I I (or gamma) interferon family. IFNG is produced by lymphocytes and activated by specific antigens or mitogens. In addition to having antiviral activity, IFNG also has important immunoregulatory functions. It is a potent activator of macrophages and has antiproliferative effects on transformed cells. It can potentiate the antiviral and antitumor effects of the type I interferons. Genetic variation in IFNG is associated with the risk of aplastic anemia (AA) which is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

• Abbreviation:

  Interferon γ, IFNG

• Synonyms:

  IFN-gamma; Ifng; Interferon gamma

• UniProt:

  P01580

• Accession Number:

  P01580

• Expression System:

  E. coli

• Tag:

  C-His

• Sequence:

  His23-Cys155

• Applications:

  Cell culture

• Endotoxin:

  < 0.01 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 98 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measure by its ability to anti-viral assay in L-929 cells infected with encephalomyocarditis (EMC) virus.The ED₅₀ for this effect is <0.5 ng/mL.The specific activity of recombinant mouse IFN gamma is approximately >2x 10⁶ IU/mg.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  16.5 kDa

• Observed Molecular Weight:

  16 kDa

• Formulation:

  Lyophilized from sterile PBS, pH 7.4.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse