Recombinant Mouse DDR2 Kinase/CD167b Protein (His Tag)

• Background:

  Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.

• Synonyms:

  CD167 antigen-like family member B; CD167b; Ddr2; Discoidin domain receptor 2; Discoidin domain-containing receptor 2; Neurotrophic tyrosine kinase; Ntrkr3; Receptor protein-tyrosine kinase TKT; Tyrosine-protein kinase TYRO10; receptor-related 3; tyro10

• UniProt:

  Q62371

• Accession Number:

  NP_072075.2

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Met 1-Arg 399

• Applications:

  ELISA

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured by its ability to bind rat tail Collagen I in a functional ELISA.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  44.0 kDa

• Observed Molecular Weight:

  80 - 90 kDa

• Formulation:

  Lyophilized from sterile PBS, pH 7.4
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse