Recombinant Human Ephrin B Receptor 1/EphB1 (C-Fc)

• Background:

  Ephrin Type-B Receptor 1 (EPHB1) is a single-pass type I membrane protein that belongs to the Ephrin-B family of receptor tyrosine kinases that is involved in embryonic nervous and vascular system development. EPHB1/EPHT2 contains two fibronectin type-III domains, one protein kinase domain and one SAM (sterile α motif) domain. EPHB1 could stimulate fibroblast motility on extracellular matrix in a kinase-dependent manner, which also correlated with its association with Grb7, an adaptor molecule implicated in the regulation of cell migration. It binds to ephrin-B1, ephrin-B2 and ephrin-B3. EPHB1 plays an important roles in diverse biological processes including nervous system development, angiogenesis, and neural synapsis formation and maturation and may be involved in cell-cell interactions in the nervous system.

• Abbreviation:

  Ephrin B Receptor 1, EphB1

• Synonyms:

  EK6; ELK; EPH Tyrosine Kinase 2; EPH-Like Kinase 6; EPHB1; EPHT2; Ephrin Type-B Receptor 1; HEK6; NET; Neuronally-Expressed EPH; Related Tyrosine Kinase; Tyrosine-Protein Kinase Receptor EPH-2; hEK6

• UniProt:

  P54762

• Accession Number:

  P54762

• Expression System:

  HEK293 Cells

• Tag:

  C-Fc

• Sequence:

  Met18-Pro540

• Field of Research:

  Signal Transduction; Cardiovascular; Neuroscience; Cancer; Stem cells

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  85.6 kDa

• Observed Molecular Weight:

  85 - 110 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human